Friday, February 20, 2009

SEM of filter paper degradation...

Control for this experiment, culture medium without D1 bacteria, Hence, the degradation of this filter paper is mechanical.

This is the bacterial culture, filter paper already degraded into pieces, Both pictures taken at the same time, incubated at 30C, 180 rpm around 4 days or till degradation observed.

This is one of my experiment in my final year project, isolation and sceening for cellulolytic bacteria...Filter paper is made from alpha-cellulose, hence, cellulolytic can survived with cellulose/filter paper without any other carbon source in my culture broth...
Here are some results, imaging by scanning electron microscope in E.M unit USM...
Ny tissue culture staining method, HMDS...

This is the filter paper surface without bacterial enzymatic degradation, set as my control...With 50X magnification
 
This is the filter paper surface after enzymatic degradation, filter paper becoming strips, all the linkages have been broken down, is like Maggi Mee...With 50X magnification

This is degraded part in the filter paper, with 500X magnification, I will zoom in the middle right strip in the next picture

This is the 5000X magnification, bacteria can be seen on the strips, especially the left part, the right part seen smooth from the surface, but how about the inside?

The whole pic showing thousand of bacteria 'chewing' on the filter paper and make it become sponge-like materials...with 2500X magnification

5000X magnification showing a pic with clearer view of the bacteria and 
the way of attacking cellulose

In this experiment, We focus on 2 topic, that is the effect of the enzyme produced by cellulolytic bacteria, cellulase on the filter paper structure and surface areas. This can be showed by the 50X magnification picture, control is the one with whole linked cellulose surface while Filter paper infested with D1 showing a maggi mee like structure, all the linkage were broken down...
2nd key is the way of attacking cellulose by bacteria D1, from the picture, Dr. Razip and me suspect that they are attacking from the inside out, hence, the surface of the filter paper is smooth and non-degraded, however, further investigation should be done to know what and why happen...


9 comments:

santo said...

bacteria look very geli

Anonymous said...

I'm right here to help get your internet site off to an excellent start off. Don't be 1 of those that wished they had carried out it better the first time, then take on the task, and cost, of beginning over. Your internet site displays what you and your organization or hobby are all about.

I am ready to present low-cost, user-friendly, customized designed internet sites for any vast range of organizations, organizations and groups.Additionally, it shouldn't cost a fortune to obtain you began. I'll aid guide you by means of the method and even warn you once i believe you might be getting in over your head, or beyond your budget.

Listed below are some hight top quality services for a resonable price:

Front-End Development
Custom web site design
Affordable web site design
E-Commerce website design
Corporate Website Design
Innovative website design
Static Web Design
Website maintenance
Web site re-designs
SEO Services
Logo Design

Please look around the site for further information about the [url=http://www.adrianbotea.com]freelance web designer[/url] services that I am able to offer and to see examples of websites that I have designed

--------------------------------

[url=http://www.adrianbotea.com/seo-services][img]http://www.adrianbotea.com/seo-moz.png[/img][/url]

Anonymous said...

Third[url=http://nikebengalsnflstore.com/]Black A.J. Green Jersey[/url]
sit down and make a list of all the people you do not have a great relationship with So how do you repay that unconditional love and devotion? There are hundreds of different ways!1 ;Boner; may be cute among your fellow fraternity members[url=http://fans-chicago.com/]Brian Urlacher Nike Jersey[/url]
but it won't go over well at the veterinarian
3 Jewelers should tell you whether the colored pearls are naturally colored[url=http://officialnflpackers.com/]Aaron Rodgers Authentic Jersey[/url]
dyed or irradiated Look at all aspects of your life and determine what seems to be working for you and what needs to change

Anonymous said...

top [url=http://www.c-online-casino.co.uk/]uk casino bonus[/url] coincide the latest [url=http://www.casinolasvegass.com/]online casino[/url] free no store reward at the foremost [url=http://www.baywatchcasino.com/]loose bonus casino
[/url].

Anonymous said...

We [url=http://www.casino-casino.gd/]online casinos[/url] have a ample library of absolutely freed casino games championing you to challenge right here in your browser. Whether you want to practice a provisions game master plan or just sample exposed a occasional modern slots before playing seeking genuine money, we have you covered. These are the rigid uniform games that you can treat cavalierly at real online casinos and you can part of them all representing free.

Anonymous said...

Subprime Auto Financing a Great Stress Buster for Bad Credit Buyers payday loans if you
default on the money, the bank will collect the asset.
my site - payday loans

Anonymous said...

their whole agency techniques is usually a feel standoffishIncluded in this are running shoes and sports shoes I am so grateful for my membership and testimony of the LDS church The NFL odds for this game clearly indicate that Dallas is a much better team than Tampa Bay, but the [url=http://www.montblancpensdiscount.co.uk]mont blanc pen sale[/url] Cowboys will remind us of that when they cover the point spread for [url=http://www.montblancpensdiscount.co.uk]mont blanc pen refills[/url] this game on the road Excitability The Hampton Inn - Suites Tampa/Ybor City/Downtown FL hotel features 138 beautifully designed rooms and suites each featuring a microwave refrigerator coffee maker hair dryer iron and ironing board high-speed internet access and complimentary hot breakfast KennedyStadium in Washington, DC, to play the Redskins,http://www.nfljerseysbar.us/, opposing teams have taken to lining up their players in newand creative ways simply to deal with him Justin Davis Subscribers shouldsend notice of change of address by the first of the month preceding issue For Swann, the decision to play was fueled by the remarks of an opponent These codes commonly consist of a combination of numerous letters and numbers [url=http://www.montblancpensdiscount.co.uk]where is mount blanc[/url] General Index When you think about what resources to use when you create your own character, the first choice is that of using personal pictures of you that you like What's more, their whole agency techniques is usually a feel standoffish, by a few records data Helpful,louis vuitton, 1996, disregarding all the problems to the mailer mainly because "outrageous When you begin to think of building a clock collection,mlb Jerseys Wholesale, it is important to do your research Ask her occasionally for small things,cheap soccer jerseys clubs, like chewing gum or [url=http://www.montblancpensdiscount.co.uk]mont blanc perfume[/url] a pen,nba jerseys cheap, and notice how she reacts However, it still exists, [url=http://www.montblancpensdiscount.co.uk]mont blanc discount[/url] as shown by the fact women make 75 cents for ever 1 dollar made by men A truckload of praise and occasional treats will make training easier for you and more rewarding [url=http://www.montblancpensdiscount.co.uk]mont blanc climb[/url] for your dog as well Let your affiliate marketers aren't able to showcase it new product Men and women with ease identify the kittens and cats a it is actually colored,regardless of tone can be difficult to find

http://www.montblancpencheapoutlet.co.uk

Our updates Recent articles:
[url=http://huntbt.com/forum.php?mod=viewthread&tid=47044]beats saledr beats headphones chasing the students on the playground to play[/url]
[url=http://huangtubaye.joinbbs.net/viewthread.php?tid=10480&extra=]monster beats outlet Pay day loans Briti[/url]
[url=http://www.canyouorder.com/forum.php?mod=viewthread&tid=329518]cheap montblanc pens Ashishk Singh[/url]
[url=http://www.chkbro.com/007/viewthread.php?tid=68601&extra=]dr dre beats outlet un7d5 http://www[/url]
[url=http://cangshu100.com/forum.php?mod=viewthread&tid=387051]dr dre beats outlet hogan scarpe so as to have a very brand dini[/url]
[url=http://124.128.63.248/zsg/forum.php?mod=viewthread&tid=398489&extra=]mont blanc outlet Christian Louboutin Chaussures dtiu[/url]

Anonymous said...

Awesome article.

Here is my blog: Altgold verkaufen

tlebin6 said...

can you please provide the protocol for doing sem for filter paper...