Friday, January 16, 2009

Bad Day 16/01/09

16th JAN 2009 not a good day for me i think...
Noon, go to lab, met Dr.Razip, discuss my problems with him, he calculate enzyme activity, teach me some theories and adjust my protocol...

Then, I went to my biotech lab to prepare my culture medium for D1 isolate for autoclaving. While waiting for autoclave, I'm going for subculturing the isolate from Agar.

After subcultured, I'm trying to do gram staining. The bad thing start from here, I'm using the fire to fix the sample on my glass slide, Then, burn the glass is what I didn;t try before, Lab assistant just told us not to do, but why... so I'm put my glass on the fire... Then, it boom! become pieces, WOW! A small piece of the hot glass drop on my hand, lagi WOW!, i push it away, but my dead skin gone, left white layer of it...

Right after my hand kena boom...

Closer look

After some time, some 'liquid' inside the skin already...swelling...

If I'm wearing a lab coat, it should not be happened, then why am I not wearing a lab coat? 
Is my mistake this time, I'm should be wearing a lab coat in the lab while doing lab work...

Ok, is broken so I have to use another galss slide for gram staining...
After did it, cant show me anything, so I redo.

3rd glass, look quite successful, but at the last stage, after I added in safranin, I drop it accidentally, and, it broken again.

4th glass, I manage to stain it, but I think the layer of the cells are too thick, I cant c anything from the slide...

4.30pm then, the medium autoclaved, so I decide to subculture D1 isolate first, After subcultured is already 5.30pm, Then i keep my stuff, planning to go pool...

For fitness test...

Interaction day 08/09 part1 - The journey to Bukit Hijau

Interaction day, an annual activity by USM lifeguard corps S & R department, hehe, S & R is Social & Recreational(but our 2nd batch captain established since 1979 , u can imagine how old is him, told us is Sex and Relax...is just a joke la btw)

Interaction day is like a orientation for new lifeguards, aim to strengthen the bonds between seniors and juniors and ex-lifeguards. During my 1st year, we went to Sungai Sedim, my 2nd year, we went to Dusun Eco, This year, we went to Bukit Hijau.

Interaction day will have its own committee, solely from S & R department (those old biscuits only)through discussion and argument for games we will play and how juniors will be played.

10th JAN 2009, 6.00am, we gathered at Padang kawad USM, and have breakfast at Khaleel, an mamak restoran opposite USM. There are cars, forgot how many, i think is 11, so we go by car pool. 
 

Gathering at padang kawad at 6am sharp, but still, somes are late...

In Khaleel, having breakfast...

2 row of long tables joining together, total around 40lifeguards...

Im in Ruo-wen's myvi, together with soon jiun and yan hwan...

with the car...

The car following us...From left, jia hao, wei ming...


taken during the 1st stop...

Beautiful pattern of the clouds...
on the way, a abandoned building, Should be nests for drug addictor...
oil palm platation - green cancer for forest... 

mosque

During the journey...

Reach the parking at Bukit Hijau...

1 car is missing because they got shortcut and they think they know the route, 
Obviously don't know the meaning of car pool and esprit d' corps...
Hope this kind of thing wont happened anymore, we wasted more than 30minutes on that... 
We gotta be more tough, more team work, be punctual, self control...
Esprit D' corps
feelings of pride and loyalty that are shared by members of a group
A common spirit of comradeship, enthusiasm, and devotion to a cause among the members of a group
consciousness of and pride in belonging to a particular group
the spirit of a group that makes the members want the group to succeed


Thursday, January 15, 2009

Filter paper SEM

Academic year 08/09, my final year in microbiology, at the same time, I have been assigned a final year project title under Assoc. Prof. Dr. Razip Samian. Screening for cellulolytic bacteria from external environment. Whole project idea is searching for bacteria that able to degrade cellulose (Oil palm wastes such as palm fronds) into glucose, then, from glucose to ethanol. For my FYP, I will concentrate on the 1st conversion, that is from cellulose to glucose. 

Why is it important?
Ethanol is the popular biofuel that contain the potential to replace our dependence on fossil. The research field is emerging, US government urge and support the scientists to develop on this, there are cars moving by using ethanol instead of gasoline. some using mix between gasoline and ethanol, some say it is called gasohol. Hence, if success to search for cheap and easy methods, we will have unlimited sources of energy from the agricultural wastes.

These are pictures of Scanning electron microscope (SEM) for filter paper degradation observation. However, No significant results showed, after discuss with Dr, we think that this is not a good way to do the test, but have to be included in my thesis also.


FT32, bacteria isolated from Kemendor, Jasin Melaka, oil palm estate, there is a crack, not sure whether is attacjed by bacterial enzyme or mechanical damage.

Filter paper, 98% of alpha-cellulose in the fibers. 

Control, without bacteria culture, This is the filter paper.
 
Another picture of filter paper surface.

Tuesday, January 6, 2009

When I'm Free...

What I did in my University life?
Can be academic, non-academic, i think 80% are non, hehe...
not a book worm, not an extrovert...
shy, quiet, 
speak as slow as possible to save energy in order to fight global warming(rubbish talk)...
not fat but not slim also(I think soon will be fit, haha!)
my course requires a lot of lab works, instead of studying, practical seems more important to us, however, the reason behind the tachnical skills need to be understood. 

So, what I did? here's some references for you to fill in your time...hehe..


Take a camera to convocation to shoot some memorial scene with your graduating seniors, then? keep the pic in your external as deepest memoris, i think the pictures will be forgotten if you not very cook with the particular senior.
Here is me, cau cieh and chin yee. cau cieh is our lifeguard senior, from industrial technology school, a strong runner, is one of the 北海四大健将! haha...



Orgnize some useful course for the uni community, such as this CPR course for basic life saving. This is our annual activities...erm, is every Sem...aim to promote CPR to the non-medical personnel esp USM students. It is also the basic requirement for bronze medallion entry (1st life saving level). Examiner (standing infront of the audiences) - Mr.Hooi, 3rd batch of USM lifeguard captain if not mistaken.



When boring, act dying...let other ppl save u...good for them as practice, good for me as i can rest...hehe...



playing with bacteria...how to play microbes in your house? try put 2 plain roti at your window, one soak with water, another one dry...see how fast the fungi can grow in your area...
I play this experiment at my 1st year where I'm staying at 3rd floor...water play an important role in life... 



side of my school, this building is the vector and parasite control management building, USM is the only Uni offer this course in South East Asia. 


Birthday celebration after general meeting, mixer for senior and junior.


Soon Wen briefing to juniors...


Swimming, USM swimming pool, 2nd home of usm lifeguards. night view.


day view.


Eating when u free... ?


playing UNO when u free...?


Nap when you are free...?


vendalism?


BBQ?


Jazz Band concert?


Going for shopping?


Watching TV?


Internet?


Monopoly to build up business power?


or FIFA..


G.O.A.Ls

Monday, January 5, 2009

My girl

September 28 is our anniversary,
September 25 is my birthday,
January 06 is her birthday.

She is a ordinary chinese girl from Tai Ping, staying in KL, currently studying in USM Penang. 
2006 July, we entered the University as fresh man, I'm taking pure biological sciences while she is taking management study. We do not know for each other.

I'm taking life saving course in my 1st academic year and passed as basic requirement to join USM lifeguard corps in the 2nd SEM 06/07.
She is learning swimming, passed the bronze medallion level and join the corps in 1st SEM 07/08.

I'm majoring in microbiology after being 2nd year student while she is majoring in international business during 2nd year. (**The chairman of this international business is Datin who lack of management skills, responsibilities and creditibility)

2nd year, we started to get to know each other... 
we start sms-ing, have lunch together, walking around in the campus, caring for each other.
not yet a official couple.

she sick one day, I worry, but nervous to show my care, she is down. 
In room, like the ants on the fire, cant sms, cant enter her room(malaysia is a very conservative country, guys cant enter girl's block)

then, she better, i treat her lunch.

after few days, cinema for our first movie - Invasion.

In the movie, chemical factors push us together in the unmatched aura of the show. 
she lie on my shoulder, my arm around her neck, cant focus on the show.

From there, we started our journey... 


 sweetest memories~

From left, sitting: Ruo wen, Edwin, me, Chin Yee, Ming Chee, Sze Yin.
From left, standing: soon jiun, james chua, lye siong, cau cieh, kevin ling.
Chinese new year reunion dinner 07/08 of lifeguard corps, as the social n recreational department head, we organize this dinner. for the reunion of juniors and seniors and ex-lifeguards.

This is me using rope throwing rescue to save a 10meters away casualty and exit casualty in life saving demonstration to pulapol.

Chin yee briefing about methods to exit casualty out of the waters to the attendants. we handle different stations to save the demonstration time for them.

From left: wei wah, hong en, chin yee, evan, min fey, teen yuan.
Taken after the life saving demonstration in USM swimming pool, the 2nd home of USM lifeguards.

In chin min's wedding dinner, with chin yee outside the ball room.

In dinner table, I just yum seng finish, tiring, haha...

My dearest... in front of de bali, going to have some drinks during chin min's bachelor's night.

Malacca, before going back to penang, riding on the Keris of malacca. RM 10 for malaysian, foreigner RM20, where is the ethic? Not very enjoying, just turn turn turn and come down.

In Johor, my home, with dad, mum, sis, xbb and our little princess: Fergie (mini pinscer)

Chin yee in 2nd fitness test, we are running a triathlon test for all the lifeguards. = ) all passed.

NAughty sapo in my lab. 
xbb in front of incubator, in my lab.

Xbb locker in lifeguard room, the locker key with voodoo from my creation, hehe...fat fat one...

The girl behind successful guy, hehe...



Happy Birthday, my little girl, I love you~